Diacetylchitobiose deacetylase
WebNov 7, 2013 · In order to develop a structure-based understanding of the chitinolytic pathway in hyperthermophilic Pyrococcus species, we performed crystallographic studies on N,N'-diacetylchitobiose deacetylases (Dacs) from Pyrococcus horikoshii (Ph-Dac) and Pyrococcus furiosus (Pf-Dac). Neither Ph-Dac nor Pf-Dac was expressed in the soluble … WebOct 28, 2024 · Deacetylase from the extremophile Pyrococcus chitonophagus has been analyzed by X-ray crystallography, small-angle X-ray scattering, differential scanning …
Diacetylchitobiose deacetylase
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WebAug 24, 2024 · This deacetylase can also use diacetylchitobiose-6-phosphate as a substrate to produce monoacetylchitobiose-6-phosphate, which is the inducer and … WebN,N'-diacetylchitobiose deacetylase (Dac) belongs to the CE-14 family and plays a role in the chitinolytic pathway in archaea by deacetylating N,N'-diacetylchitobiose (GlcNAc2), which is the end ...
Webdiacetylchitobiose deacetylase (Dac), which was firstly derived from hyperthermophilic bacteria, to demonstrate the advantages of our method. With the optimized fabrication steps, our hydrogel WebA linear polysaccharide of beta-1->4 linked units of ACETYLGLUCOSAMINE. It is the second most abundant biopolymer on earth, found especially in... Explore the latest full-text research PDFs ...
WebAug 4, 2024 · Gene ID: 13302188, updated on 4-Aug-2024. Summary Other designations. PIG-L family deacetylase. GeneRIFs: Gene References Into Functions. Crystal structure of N,N'-diacetylchitobiose deacetylase from Pyrococcus furiosus has been reported. WebWe constructed a recombinant Escherichia coli and Bacillus subtilis strains as efficient whole-cell biocatalysts via expression of diacetylchitobiose deacetylase (Dacph) from Pyrococcus horikoshii. Although both strains were biocatalytically active, the performance of B. subtilis was better.
WebJul 1, 2013 · The purified Pyrococcus Dacs were active and thermostable up to 85 °C. The crystal structures of Ph-Dac and Pf-Dac were determined at resolutions of 2.0 Å and 1.54 Å, respectively. The Pyrococcus Dac forms a hexamer composed of two trimers. These Dacs are characterized by an intermolecular cleft, which is formed by two polypeptides in the ...
WebIn this study, semi-rational design based on site-directed saturation mutagenesis and surface charge modification was used to improve the catalytic efficiency of the … canal flow music onlineWebJul 16, 2004 · The hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 possesses chitinase (Tk-ChiA) and exo-β-d-glucosaminidase (Tk-GlmA) for chitin degradation; the former produces diacetylchitobiose (GlcNAc 2) from chitin, and the latter hydrolyzes chitobiose (GlcN 2) to glucosamine (GlcN).To identify the enzyme that … canal festival tonawanda nyWebSo far chitin deacetylase from fungi and insects, chitooligosaccharide deacetylase (NodB) from Rhizobium, and bacterial GlcNAc6P deacetylase have been known as catalytically … fisher price barnyard bounce houseWebAug 23, 2024 · We selected the enzyme diacetylchitobiose deacetylase (Dac), which was firstly derived from hyperthermophilic bacteria, to demonstrate the advantages of our method. With the optimized fabrication steps, our hydrogel showed ~87% Dac immobilization efficiency and excellent stability against heating, dehydrating, long-time … canal flooringWebOct 28, 2024 · Deacetylase from the extremophile Pyrococcus chitonophagus has been analyzed by X-ray crystallography, small-angle X-ray scattering, differential scanning calorimetry, isothermal titration calorimetry and NMR to determine its structure, thermodynamics and enzymatic properties ... can alfoil go in the microwaveWebDec 1, 2024 · Diacetylchitobiose deacetylase (Dac) from Pyrococcus horikoshii can realize the one-step production of glucosamine (GlcN). The efficient expression and secretion of Dac play a central role in the green production of GlcN. In this study, Bacillus subtilis WB600 was used as the expression host. Firstly, we screened 12 signal … fisher-price barnyard rhythm and moosWebJun 28, 2024 · Diacetylchitobiose deacetylase (Dac) from Pyrococcus horikoshii can realize the one-step production of glucosamine (GlcN). The efficient expression and secretion of Dac play a central role in the ... fisher price baseball bat