Phnshp45
Webb23 feb. 2024 · TEXT. The discovery of the plasmid-mediated colistin resistance gene mcr-1 has captured global attention. Unlike colistin resistance raised by chromosomal mutations, the plasmid carrying the mcr-1 resistance gene can be rapidly disseminated through horizontal gene transfer. Since the first report of plasmid pHNSHP45 carrying the mcr-1 … Webb21 mars 2024 · The emergence and spread of antibiotic resistance genes (ARGs) have imposed a serious threat on global public health. Horizontal gene transfer (HGT) via plasmids is mainly responsible for the spread of ARGs, and conjugation plays an important role in HGT. The conjugation process is very active in vivo and its effect on the spreading …
Phnshp45
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Webb24 maj 2016 · In particular, the pHNSHP45 3 is the only one mcr-1-carring plasmid with known genome sequence prior to our study. Unlike the other mcr-1 -positive plasmid … WebbThe PCR based mapping of mcr-1 genetic context in our isolates revealed that almost all amplified genes were similar to pHNSHP45, except the truncated tnpA gene, missing IsApl1. Globally, plasmid analysis of various Incompatibility types has shown that IsApl1 is usually absent in IncX4 type plasmid while in Incl2 type plasmid, it is either absent or …
Webb27 juni 2024 · For instance, a 222.3-kb section of pWJ1 (85.4% of the total sequence) exhibited high similarity to the corresponding region of the 251-kb plasmid pHNSHP45-2 … Webb开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆
Webb图4 大肠杆菌 SHP45 携带 mcr-1 基因质粒 pHNSHP45 的结构. 3. 多黏菌素耐药现状. 由于质粒介导的 mcr-1 基因可以在不同菌株间进行水平传播，致使动物源性多黏菌素类药物耐 … Webbthe plasmid (pHNSHP45) contained in this Chinese strain. Moreover, this plasmid was determined to be 100% identical to a plasmid (p13P477T-7) belonging to an Escherichia …
Webb开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆
WebbDOI: 10.1016/S1473-3099(16)00010-4 Corpus ID: 36290610; Colistin resistance gene mcr-1 and pHNSHP45 plasmid in human isolates of Escherichia coli and Klebsiella … gpt in frenchWebbCRISPR/Cas9 system can efficiently eliminate plasmid from E. coli, the control vector pCas9, and the engineered vector pCas9-m1 were transformed into competent E. coli … gpt in fullWebb15 mars 2024 · Plasmid pHNSHP45 (GenBank accession number: KP347127) was introduced into Escherichia coli BW25113 by conjugation using E. coli C600/pHNSHP45 as the donor . The strains carrying pHNSHP45 and its derivatives (n = 3) were grown in LB broth overnight at 37°C, then diluted 1:200 to fresh medium and shaking (250 rpm) at … gpt in informaticaWebb18 nov. 2015 · To identify the gene responsible for polymyxin resistance, pHNSHP45 was completely sequenced, which revealed a plasmid 64 015 bp in size with an average GC content of 43·0%. pHNSHP45 contains 60 predicted open reading frames and possesses a typical IncI2-type backbone (57 624 bp) encoding replication, horizontal transfer, … gpt infraprojects limited addressWebb27 juni 2024 · For instance, a 222.3-kb section of pWJ1 (85.4% of the total sequence) exhibited high similarity to the corresponding region of the 251-kb plasmid pHNSHP45-2 from E. coli strain SHP45, from which the first mcr-1 gene was identified . pWJ1 and pHNSHP45-2 had 12 resistance genes in common (Fig. 1A), while pWJ1 appeared to … gpt insulation gasketWebb24 maj 2016 · In particular, the pHNSHP45 3 is the only one mcr-1-carring plasmid with known genome sequence prior to our study. Unlike the other mcr-1 -positive plasmid pKH457-3-BE 2 that is from the cattle isolate in Belgium and has the plasmid signature of IncP, both pHNSHP45 3 and pWH12 are hosted by E. coli isolated from swine in China, … gpt in life insuranceWebb19 dec. 2016 · Compared with the original mcr-1-bearing IncHI2 plasmid pHNSHP45-2, 23 three regions in which antibiotic resistance genes were observable were found to exhibit structural differences (Figure S2). First, the IS Apl1 - mcr-1 - orf region located between terF and the hypothetical CDS was absent in pHYEC7-IncHI2, indicating that mcr-1 could … gpt input length