WebDec 1, 2010 · pEBG2t plasmids encoding GST alone, GST-ERK5(WT) (human ERK5 wild-type) or GST-ERK5(AEF) (ERK5 mutated at Thr218/Glu/Tyr220 to Ala218/Glu/Phe220) were a generous gift of Dr. S. Arthur (MRC Phosphorylation Unit, Dundee, UK). ... an antibody that recognises unphosphorylated and phosphorylated ERK5 equally well, or with a phospho … WebPhospho-ERK5 (Thr218/Tyr220) Antibody (Cell Signalling Technology, #3371). 3. ... The same lysates were also run on a 7 % acrylamide Phos-Tag™ gel and probed with the total ERK5 antibody. The phospho-ERK5 antibody identified a band at approximately 102 kDa, with a small increase in phosphorylation evident at 30, 60, and 180 min. The use of ...
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WebAug 9, 2013 · While mitogen activated protein kinases (MAPKs) are known to promote ligand-independent cell growth, the role of the MEK5-ERK5 pathway in the progression of clinical breast carcinoma remains poorly understood. Here, we demonstrated increased ERK5 activation in 30 of 39 (76.9%) clinical tumor samples, as well as across breast … the pinnacle st john\u0027s
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WebNov 16, 2008 · Phosphorylation of ERK5 was determined by using a phospho-specific ERK5 antibody, which recognizes ERK5 only when phosphorylated at Thr218/Tyr220. Ang II rapidly induced ERK5 phosphorylation within 45 s, with peak phosphorylation between 4 and 16 min that was sustained for hours; the phosphorylation returned to the baseline level at 90 min … WebRabbit Polyclonal antibody to ERK5 (phospho Thr218/Tyr220) IgGy Antibody Selector – Quickly search hundreds of thousands of antibodies available for purchase from VWR by … WebeFileMA allows filers to easily open court cases and e-file documents to participating courts anytime and from anywhere — 24 hours a day, seven days a week, 365 days a year. E-filing … side effects of a sugar rush