WebTo test the transfection efficiency of the reverse transfection approach, a luciferase-encoding plasmid was “reverse” transfected into HEK293, Hep G2 and Jurkat cells using FuGENE® 6, FuGENE® HD, Trans IT®-LT1 and Lipofectamine™ 2000 transfection reagents. Luciferase activity was measured after 24 hours (Figures 2–4). WebMay 15, 2014 · Although Lipofectamine and Polyfect have manufacturer optimized protocols, ... 50% (v/v) glycerol, 1 m M dithiothreitol and 0.1 m M EDTA) and stored at a concentration of 15 ...

Regulation of eIF4E guides a unique translational program to …

Webgated, and Vero, 293, HeLa, 2fTGH, and 2fTGH SV5-V(CPI-) cells were maintained as described (14). Transfections were carried out by using Lipofectamine (Invitrogen), … signs of infection in brain

BBRC - Cardoso Lab

WebStructural basis of pre-existing immunity to the 2009 H1N1 pandemic influenza virus Rui Xu1*, Damian C. Ekiert1*, Jens C. Krause2, Rong Hai3, James E. Crowe, Jr.2, Ian A. Wilson1,4† 1Dept. of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA 2Dept. of Pediatrics and of Microbiology and … Web4 lg pUHrT62-1M2 using PolyFect as a transfection reagent (Qiagen, Basel, Switzerland), according to the manufacturers protocol. Non-transfected controls were treated without PolyFect DNA mix. Twenty-four hours after the transfection, the supernatant was removed and fresh cell culture medium containing 1 lg/ml Dox, 4 mM L-carnitine, WebSep 30, 2002 · Recently we reported that neurotensin-SPDP-poly-L-lysine (NT-vector) is able to bind plasmid DNA (NT-polyplex) and polyfect cells expressing the high-affinity neurotensin receptor (NTRH) although with low transfecting efficiency: in vitro, 6.5+/-1.5%, and in vivo, 5+/-4%. In this work, we attempted … therapeutic storywriting