Pshuttle-gfp
WebVector pShuttle is used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired. Max insert size = 7.5kb with pAdEasy1 and 10.2kb with … WebDec 1, 2009 · The formed plasmids, pShuttle-GFP-TPE and pShuttle-GFP-D19K, respectively, were the final shuttles. These two plasmids were recombined with pAdEasy-1 to generate oncolytic Ad plasmids carrying the GFP gene. 3.2. Armed oncolytic Ads were rescued in packaging cells Ads were rescued in 293 cells by transfection of PacI-linearized Ad …
Pshuttle-gfp
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WebpShuttle-IRES-hrGFP-1 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially … WebJun 23, 2011 · The recombinant adenovirus carrying FHL1 or green fluorescent protein (GFP) coding sequence was generated using the Ad-Easy system according to the manufacturer's protocol (Stratagene). Briefly, FHL1 or GFP cDNA was cloned into the pShuttle-CMV vector to create pShuttle-FHL1 or pShuttle-GFP. The pAdEasy-1 vector, …
WebRecombinant adenoviruses are a versatile tool for gene delivery and expression. Choose pAdEasy-1 vector or any of these three shuttle vectors, pShuttle, pShuttle-CMV, or pShuttle-CMV-lacZ vector, to clone DNA of interest with the AdEasy adenoviral vector system. Several features of adenovirus biology have made such viruses the vectors of choice ... WebpShuttle-IRES-hrGFP-1 Vector by Agilent Technologies Manufacturer Agilent Technologies Model: 240081 Be the first to review this product Recombinant adenoviruses are a versatile tool for gene delivery and expression.
WebJan 7, 2024 · Product Name : pShuttle article : bacterial resistance : Kanamycin cloning : growth notes : Vector pShuttle is used for expression of transgenes under a chosen (non … WebReviews. Product Specs. Item pShuttle-IRES-hrGFP-1. Company Agilent Technologies. Price Supplier Page View Company Product Page. Catalog Number 240081. Host mammalian. …
WebNov 2, 2015 · The Ad vector pShuttle-CMV (Stratagene) was rendered replication defective by deletion of the E1 and E 3 genes [ 23 ]. The GFP coding sequence was amplified from the pEGFP-N1 vector (Clontech) using the primer pair 5′-ATGGTGAGCAAGGGCGAG-3′ and 5′-TTACTTGTACAGCTCGTCC-3′.
http://www.labbase.net/Supply/SupplyItems-4229347.html scratch sapoWebpShuttle-IRES-hrGFP-2. Cat.No. OVT2909. Host Cell. Mammalian cells, Escherichia coli. Product Type. Vector. Promoter. scratch sandwich laredo txWebGFP) and the shuttle vectors (pShuttle) were digested with NotI and KpnI. The 412bp (U6-Acrp30 cDNA) and 6.6 kb (pShuttle) frag-ments were collected and ligated. The shuttle plasmids (pShut-tle-U6-Acrp30 and pShuttle-GFP) were linearized by Pme I and transformed into BJ5183-AD-1 cells (harbor pAdEasy-1) by elec-troporation. scratch sandwich laredo facebookWebBriefly, pShuttle-GFP- CTRP3 was constructed by cloning the target gene CTRP3 into pShuttle-GFP-CMV. After sequence confirmation, pShuttle-GFP- CTRP3 was transferred into the recombinant adenovirus frame vector of pADxsi. The pADxsi-GFP-CTRP3 adenovirus vector was then amplified in 293 cells, and the viral titer was measured. scratch sarvente testWebVector Name pShuttle-IRES-hrGFP-1; Specifications/Features Vector Size: 8.9 kb. Add to Compare List. Agilent Technologies. 5301 Stevens Creek Blvd Santa Clara, California 95051. United States Phone: +1 877-424-4536. Company … scratch sandwich company laredo texasWebpShuttle-CMV-IRES-GFP Sequences (1) Addgene Sequences: Full (1) Full Sequences from Addgene (1) Based on next-generation sequencing (NGS) results where indicated … scratch sanicWebA GGGGCC hexanucleotide repeat expansion in intron 1 of chromosome 9 open reading frame 72 (C9ORF72) gene is the most common genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal ... scratch saut