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Psin-ef2 vector

WebApr 20, 2012 · Fig. 7 outlines the vector (pSin-EF2-TK-Puro), with TK driven by the EF1α promoter. hES H9 cells were treated with BMP4 , allowing for trophoblast differentiation. Cells were infected with either the SSEA4 or CD24 antibody conjugated m 168 pseudotyped virus bearing the pSin-EF2-TK-Puro lentiviral vector, five days post BMP4 addition and ... WebNov 17, 2024 · GFP-human Med15 and TagRFP-human DYRK3 fusion protein were subcloned into the lentiviral expression vector pSin-EF2 by ClonExpress II One Step Cloning Kit (Vazyme Biotech, C112). Primers used to clone lentiviral vectors are provided in Additional file 6: Table S3. The human embryonic kidney 293T cell line was used as a host …

Addgene: pSin-EF2-Nanog-Pur

WebAug 14, 2015 · zt085 腺病毒载体系统 pAd/BLOCK-iT-DEST RNAi Gateway Vector Invitrogen ... zt1273 pSin-EF2-puro-OCT4 . zt1274 pSIREN-retroQ . zt1275 psiSTRIKE . zt1276 pST1347-cas9-D10A . zt1277 PT/neo(2200) zt1278 PT2/GFP(2000) zt1279 PT2/mCherry(321) zt1280 PT2/RMCE-puDTK(1500) ... WebFeb 3, 2024 · The AKR1B10 CDS (coding DNA sequence) was cloned into the lentiviral expression vector pSIN-EF2-Puro (hereinafter referred to as pSIN-EF2) to construct the lentiviral vector pSIN-EF2/AKR1B10. The method to establish stable AKR1B10-expression cell line has been described previously 23. AKR1B10-shRNA stable transfection using … ppm in numbers https://enlowconsulting.com

Addgene: pSin-EF2-Sox2-Pur Sequences

WebJan 22, 2015 · The pri-miR-34c sequence was cloned into the lentiviral plasmid pSin-EF2-puromycin (Addgene, Cambridge, MA, USA); pSin-EF2-miR-34c or negative control empty pSin-EF2 vector was then co-transfected ... WebFull Sequences from Depositor (1) Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies between … pp minority\u0027s

Formation of nuclear condensates by the Mediator complex …

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Psin-ef2 vector

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WebSep 14, 2024 · The empty vector pSin-EF2-Pur was used as a control. The plasmids were verified by sequencing. For stable knockdown of ELMO1, GV248-puro [Shanghai Genechem Co., Shanghai, China] containing hairpin sequence targeting ELMO1 or a control plasmid [control hairpin] was co-transfected with psPAX2 and pMD2G [Addgene, Cambridge, MA, … WebJul 6, 2024 · Although pSIN-EF2-(Pou4f3 or GFI1)-Pur and pLV-ATOH1-IRES-eGFP belong to the third-generation of lentiviral transfer vectors [36,37], pSIN-EF2-Pou4f3-Pur and pSIN …

Psin-ef2 vector

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WebB: Schematic construction of vectors encoding soluble scFv C1 and scFv C1-N1N2. The amber stop codon between the scFv and gene III in pHEN 2 was removed by mutagenesis … http://www.labbase.net/Supply/SupplyItems-4229347.html

WebJan 8, 2024 · The pSin-EF2-puro-ARNTL, pSin-EF2-puro-vector and psin-EF2-puro-CDK5 plasmids were obtained from Land. Hua Gene Biosciences (Guangzhou, China), and the psin-EF2-puro-vector plasmid served as a control. To generate stable cell lines, lentivirus packing expression plasmids were co-transfected into 293 T cells for 48 h. Then, SUNE1 … WebJan 7, 2024 · product information Catalog Number : 16579 Product Name : pSin-EF2-Oct4-Pur article : bacterial resistance : Ampicillin cloning : growth notes : The psPAX2 …

WebMay 11, 2024 · abstract type AbstractPolynomial end """ Polynomial <: AbstractPolynomial Polynomials written in the canonical form --- Polynomial(v::T) where T<:Union{Vector{<:Real}, NTuple{<:Any, <:Real}}) Construct a `Polynomial` from the list of the coefficients. The coefficients are assumed to go from power 0 in the ascending order. WebMay 15, 2024 · 42EF-P2MPB-F4 Sensor Block 3D CAD Model Library GrabCAD. Join 9,340,000 engineers with over 4,840,000 free CAD files Join the Community. The CAD files …

WebAddgene inc psin ef2 modulation vector Psin Ef2 Modulation Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. …

WebJan 7, 2024 · Product Name : pSin-EF2-Sox2-Pur article : bacterial resistance : Ampicillin cloning : growth notes : The psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector. origin : 37 pi : plasmid copy : SINF-EF-G (from Dr. Robert Hawley, George Washington University) was modified to make the lentiviral backbone. ppm in opticsWebpSin (Search Vector Database) Vector type Synthetic Biology Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) NEB Stable Copy number Low Copy Gene/Insert Gene/Insert name Sindbis Glycoproteins Cloning Information Cloning method Unknown Resource Information Supplemental Documents ppm in pollutionWebThe empty pSin-EF2 vector (lenti-vector) was used as a control. Observation of morphologic changes. The cells were seeded into six-well culture (Corning Incorporated, Corning, NY, USA) plates and transferred with miR-16 or miR-Ctrl. After incubation for 24 hours, the cellular morphology was observed by phase contrast microscope (Olympus America ... ppm in power supplyWebLa fusariose vasculaire du cyclamen est une maladie causée par le champignon tellurique Fusarium oxysporum f. sp. cyclaminis. Elle est considérée comme l’une des maladies les plus graves du cyclamen et se traduit par des pertes atteignant jusqu’à 50 % … ppm in placeWebSystemic delivery of pSin-EF2-ELMO1-Pur facilitates continuous ELMO1 expression in colonic tissues. [A] Immunohistochemical staining of ELMO1 in the colons of untreated mice or mice treated with... ppm insecticidasWeb伟通生物现成基因表达载体库开放供应伟通生物根据基因功能研究需要,制备和积累近千个基因表达载体。其中大基因的序列都经过测序验证,与ncbi上的注册序列保持一致。这些载体既可以直接作为基因功能研究使用,也可以作为新构建载体的模板。伟通将部分常用的,不涉及第三方知识产权的表达 ... ppm inputWebThe psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector. These plasmids were created by your colleagues. Please acknowledge the Principal … ppm in printing